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tim3  (R&D Systems)


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    Structured Review

    R&D Systems tim3
    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
    Tim3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tim3/Human+TIM-3+Antibody/bio_rxiv__64898__2026__03__27__714741-48-9-11
    Average 94 stars, based on 7 article reviews
    tim3 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas"

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas

    Journal: bioRxiv

    doi: 10.64898/2026.03.27.714741

    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
    Figure Legend Snippet: (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Techniques Used: Staining, Expressing

    Related Articles

    other:

    Article Title: Dexamethasone potentiates chimeric antigen receptor Tcell persistence and function by enhancing IL-7R expression
    Article Snippet: After 7 days, CD3/CD28 Dynabeads beads were removed using a DynaMag-5 Magnet (Invitrogen, 12303D) and 7 105 cells/mL were plated in RPMI 1640/10% FBS, 50 U/mL rhIL-2, and 0.5 ng/mL rhIL-15 to rest for 48 h. CAR T cells were cultured for 16–24 days before being used in in vitro experiments or being frozen in CryoStor (Biolife Solutions, 205102) for mouse experiments.

    Article Title: Designed improvement to T-cell immunotherapy by multidimensional single cell profiling
    Article Snippet: Fluorescently labeled anti-CD107a antibodies (clone H4A3) at 1.6 μL/well, together with GolgiStop (BD Biosciences) at 0.7 μL/mL was added to the co-culture to stain for degranulating cells.

    Article Title: Dexamethasone Potentiates Chimeric Antigen Receptor T Cell Persistence and Function by Enhancing IL-7Rα Expression.
    Article Snippet: Where indicated, media was also supplemented with IL-7 (R&D 318 Systems, 207-IL-025) on days 9, 12, and 14.

    Staining:

    Article Title: Dexamethasone Enhances CAR T Cell Persistence and Function by Upregulating Interleukin 7 Receptor
    Article Snippet: Where indicated, IL-7 (R&D Systems, 207-IL-025) was supplemented to the media on Days 9, 12, and 14. .. For surface staining, cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) to CD3 (BD Bioscience, 563109, 557832), CD4 (BD Bioscience, 557852, 340133), CD8 (BD Biosciences, 348793), CD62L (BD Biosciences, 341012), CD127 (Biolegend, 351319), EGFR (Biolegend, 352906), LAG3 (LSBio, LS-B2237), PD1 (Invitrogen, 47-2799-42), TIM3 (R&D Systems, FAB2365P), CD45RA (BD Biosciences, 555488), CD45RO (BD Biosciences, 561137), CD45 (BD Biosciences, 340665), CD107a (BD Biosciences, 555800). .. Cells were resuspended in FACS buffer, which consists of HBSS (Gibco, 14175095), 2% FBS and NaN3 (Sigma, S8032), and incubated with antibodies at 4°C in the dark.

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas
    Article Snippet: Immunohistochemistry was performed on formalin-fixed paraffin-embedded (FFPE) tissue from 9 independent ALK+ ALCL patients using standard protocols on a Discovery-XT staining device (Ventana). .. Following heat-mediated antigen retrieval, consecutive sections were stained for TIM3 (MAB23652, R&D Systems) and CD30 (M0751, Agilent Dako) both at a 1:50 dilution. ..

    Incubation:

    Article Title: Dexamethasone Enhances CAR T Cell Persistence and Function by Upregulating Interleukin 7 Receptor
    Article Snippet: Where indicated, IL-7 (R&D Systems, 207-IL-025) was supplemented to the media on Days 9, 12, and 14. .. For surface staining, cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) to CD3 (BD Bioscience, 563109, 557832), CD4 (BD Bioscience, 557852, 340133), CD8 (BD Biosciences, 348793), CD62L (BD Biosciences, 341012), CD127 (Biolegend, 351319), EGFR (Biolegend, 352906), LAG3 (LSBio, LS-B2237), PD1 (Invitrogen, 47-2799-42), TIM3 (R&D Systems, FAB2365P), CD45RA (BD Biosciences, 555488), CD45RO (BD Biosciences, 561137), CD45 (BD Biosciences, 340665), CD107a (BD Biosciences, 555800). .. Cells were resuspended in FACS buffer, which consists of HBSS (Gibco, 14175095), 2% FBS and NaN3 (Sigma, S8032), and incubated with antibodies at 4°C in the dark.

    Bioprocessing:

    Article Title: Dexamethasone Enhances CAR T Cell Persistence and Function by Upregulating Interleukin 7 Receptor
    Article Snippet: Where indicated, IL-7 (R&D Systems, 207-IL-025) was supplemented to the media on Days 9, 12, and 14. .. For surface staining, cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) to CD3 (BD Bioscience, 563109, 557832), CD4 (BD Bioscience, 557852, 340133), CD8 (BD Biosciences, 348793), CD62L (BD Biosciences, 341012), CD127 (Biolegend, 351319), EGFR (Biolegend, 352906), LAG3 (LSBio, LS-B2237), PD1 (Invitrogen, 47-2799-42), TIM3 (R&D Systems, FAB2365P), CD45RA (BD Biosciences, 555488), CD45RO (BD Biosciences, 561137), CD45 (BD Biosciences, 340665), CD107a (BD Biosciences, 555800). .. Cells were resuspended in FACS buffer, which consists of HBSS (Gibco, 14175095), 2% FBS and NaN3 (Sigma, S8032), and incubated with antibodies at 4°C in the dark.

    Article Title: Dominant negative TGFβ receptor II and truncated TIM3 enhance the antitumor efficacy of CAR-T-cell therapy in prostate cancer.
    Article Snippet: PSMA-PE antibody (342507, Biolegend) was used to detect the percentage of prostate cancer cells that are positive for PSMA expression. .. Untransduced (UTD) T cells, PSMA-CAR-T cells, and DT-PSMA-CAR- L. Tang et al. International Immunopharmacology 124 (2023) 110807 L. Tang et al. International Immunopharmacology 124 (2023) 110807 L. Tang et al. International Immunopharmacology 124 (2023) 110807 T cells were co-cultured with prostate cancer cells at ratios of 1:1, 5:1, and 10:1 for 24 h.The T-cell culture medium was supplemented with appropriate concentrations (0 ng/ml, 5 ng/ml, 20 ng/ml, and 50 ng/ml) of TGF-β (P01137, Suzhou Novoprotein Scientific) and/or (0 μg/ml, 5 μg/ml, 10 μg/ml, and 20 μg/ml) TIM3 activating monoclonal antibodies (MAB23651, R&D Systems) from the day before coculture to the end of coculture. ..

    Article Title: Evaluation of the Elements of Short Hairpin RNAs in Developing shRNA-Containing CAR T Cells
    Article Snippet: .. Fluorochrome-conjugated monoclonal antibodies are CD3 (BD Bioscience, San Jose, CA, USA, 563109, 557832), CD4 (BD Bioscience, 557852, 340133), CD8 (BD Biosciences, 348793), CD62L (BD Biosciences, 341012), CD127 (Biolegend, San Diego, CA, USA, 351319), EGFR (Biolegend, 352906), LAG3 (LSBio, Seattle, DC, USA, LS-B2237), PD1 (Invitrogen, Waltham, MA, USA, 47-2799-42), TIM3 (R&D Systems, FAB2365P), CD45RA (BD Biosciences, 555488), CD45RO (BD Biosciences, 561137), CD45 (BD Biosciences, 340665), CD107a (BD Biosciences, 555800), CD45 (BD Biosciences, 340665), or CD19 (Life Technologies, Carlsbad, CA, USA, MHCD1905). ..



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    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
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    Image Search Results


    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Journal: bioRxiv

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas

    doi: 10.64898/2026.03.27.714741

    Figure Lengend Snippet: (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Article Snippet: Following heat-mediated antigen retrieval, consecutive sections were stained for TIM3 (MAB23652, R&D Systems) and CD30 (M0751, Agilent Dako) both at a 1:50 dilution.

    Techniques: Staining, Expressing